@article{oai:hokuriku.repo.nii.ac.jp:00000654,
 author = {油野, 友二 and 神田, 七瀬 and 澤井　, 咲月 and 得能　, 友実 and 松尾　, 奈留美 and 松下, 愛 and 吉川　, 葵 and Yuno, Tomoji and Kanda, Nanase and Sawai, Satsuki and Tokunou, Yumi and Matsuo, Narumi and Matsushita, Ai and Yoshikawa, Aoi},
 issue = {51},
 journal = {北陸大学紀要, Bulletin of Hokuriku University},
 month = {Sep},
 note = {Quality control in the process of preparation for urinary sediment samples is 
important for accurate diagnosis. In Japan, hematuria is defined as the presence of 
occult blood and five or more red blood cells per high power field in urinary sediment 
examination. The Japanese guidelines on urinary sediment examination procedures 
state that a urine sample should be centrifuged for 5 minutes at 500×g in the 
preparation process. Consequently, remaining components in the supernatant after 
centrifugation, especially suspended red blood cells, have been reported. However, 
changes in remaining red blood cells have not been investigated under different 
centrifugal conditions. We have investigated the percentage of remaining red blood cells 
in the supernatant, an issue in the guidelines on urinary sediment examination 
procedures, using artificial hematuria. 
 Experiment 1: To investigate the percentage of remaining red blood cells with 
different centrifugal forces, we prepared two types of artificial hematuria with pseudo -
urine made of saline and that made of 5% dextrose in acetated Ringer’s solution and 
examined the percentage of remaining red blood cells in the supernatant after 
centrifugation at 400 to 900×g. Although 3%-5% of the red blood cells remained at 500 
×g specified by the standard guideline, the percentage decreased to 2.0% or less at 700 
×g or higher. 
xperiment 2: Regarding the percentage of remaining red blood cells at different 
hours, the percentage was 6.87% for 5 minutes, 0.38% for 10 minutes, and 0.23% for 15 
minutes at 500×g in artificial hematuria with pseudo-urine made of saline. 
 This study suggests that a certain percentage of red blood cells is suspended in the 
supernatant by following the current Japanese guidelines on urinary sediment 
examination procedures, which could affect the number of red blood cells in urinary 
sediment examination.},
 pages = {135--141},
 title = {標本の精度管理から考える尿沈渣検査の課題 －標本作製における相対的遠心力の影響－},
 year = {2021},
 yomi = {ユノ, トモジ and カンダ, ナナセ and サワイ, サツキ and トクノウ, ユミ and マツオ, ナルミ and マツシタ, アイ and ヨシカワ, アオイ}
}