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Stereospecific and Regioselective Hydrolysis of Cannabinoid Esters by ES46.5K, an Esterase from Mouse Hepatic Microsomes, and Its Differences from Carboxylesterases of Rabbit and Porcine Liver
https://hokuriku.repo.nii.ac.jp/records/540
https://hokuriku.repo.nii.ac.jp/records/5406192e06a-6939-4d11-9075-bff646364a4c
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
Biological & Pharmaceutical Bulletin 2005.09 (140.3 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2018-07-26 | |||||
| タイトル | ||||||
| タイトル | Stereospecific and Regioselective Hydrolysis of Cannabinoid Esters by ES46.5K, an Esterase from Mouse Hepatic Microsomes, and Its Differences from Carboxylesterases of Rabbit and Porcine Liver | |||||
| タイトル | ||||||
| タイトル | Stereospecific and Regioselective Hydrolysis of Cannabinoid Esters by ES46.5K, an Esterase from Mouse Hepatic Microsomes, and Its Differences from Carboxylesterases of Rabbit and Porcine Liver | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | ES46.5K | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | carboxylesterase | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | regioselective hydrolysis | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | stereospecific hydrolysis | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | cannabinoid ester | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Watanabe, Kazuhito
× Watanabe, Kazuhito× Matsunaga, Tamihide× Kimura, Toshiyuki× Funahashi, Tatsuya× Yamaori, Satoshi× Shoyama, Yukihiro× Yamamoto , Ikuo |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The properties of ES46.5K, an esterase from mouse hepatic microsomes, were compared with those of carboxylesterases from rabbit and porcine liver. The inhibitory profile with a serine hydrolase inhibitor (bis-p-nitrophenylphosphate) and detergents (sodium dodecylsulfate, Emulgen 911) was different between ES46.5K and the carboxylesterases. Bis-p-nitrophenylphosphate (0.1 mM) markedly inhibited the catalytic activity of the carboxylesterases but not that of ES46.5K. Emulgen 911 (0.05—0.25%) inhibited the catalytic activity of the carboxylesterases, whereas the detergent conversely stimulated that of ES46.5K by 150%. The two carboxylesterases catalyzed the hydrolysis of acetate esters of tetrahydrocannabinol (THC) analogues with different side chain lengths (C1—C5), although ES46.5K showed marginal activity only against the acetate of Δ8-tetrahydrocannabiorcol, a methyl side chain derivative of Δ8-THC. ES46.5K hydrolyzed cannabinoid esters stereospecifically and regioselectively. The esterase hydrolyzed 8α-acetoxy-Δ9-tetrahydrocannabinol (8α-acetoxy-Δ9-THC, 5.62 nmol/min/mg protein), while the enzyme did not hydrolyze 8β-acetoxy-Δ9-THC, 7α-acetoxy-, and 7β-acetoxy-Δ8-THC at all. In contrast, the carboxylesterases from rabbit and porcine liver hydrolyzed 8β-acetoxy-Δ9-THC efficiently but not 8α-acetoxy-Δ9-THC. ES46.5K hydrolyzed side chain acetoxy derivatives of Δ8-THC at the 3′- and 4′-positions, and a methyl ester of 5′-nor-Δ8-THC-4′-oic acid. The enzyme, however, could not hydrolyze methyl esters of Δ8- and Δ9-THC-11-oic acid, while both carboxylesterases hydrolyzed side chain acetoxy derivatives of Δ8-THC and three methyl esters of THC-oic acids. These differences in stereospecificity and regioselectivity between ES46.5K and carboxylesterases suggest that the configurations of important amino acids for the catalytic activities of these enzymes are different from each other. | |||||
| 書誌情報 |
en : Biological & Pharmaceutical Bulletin 巻 28, 号 9, p. 1743-1747, 発行日 2005-09 |
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| 出版者 | ||||||
| 出版者 | 公益社団法人日本薬学会 | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0918-6158 | |||||
