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標本の精度管理から考える尿沈渣検査の課題 －標本作製における相対的遠心力の影響－
https://doi.org/10.15066/00000611
https://doi.org/10.15066/000006116afad3a5-4588-4203-a1a9-483e50c0dd08
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
hoken01 (1.0 MB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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| 公開日 | 2021-09-30 | |||||
| タイトル | ||||||
| タイトル | 標本の精度管理から考える尿沈渣検査の課題 －標本作製における相対的遠心力の影響－ | |||||
| タイトル | ||||||
| タイトル | An Issue in Urinary Sediment Examination from the Perspective of Quality Control of Samples － The Effect of Relative Centrifugal Force in Sample Preparing － | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Urinary sediment examination | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Hematuria | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Red blood cells | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| ID登録 | ||||||
| ID登録 | 10.15066/00000611 | |||||
| ID登録タイプ | JaLC | |||||
| 著者 |
油野, 友二
× 油野, 友二× 神田, 七瀬× 澤井 , 咲月× 得能 , 友実× 松尾 , 奈留美× 松下, 愛× 吉川 , 葵× Yuno, Tomoji× Kanda, Nanase× Sawai, Satsuki× Tokunou, Yumi× Matsuo, Narumi× Matsushita, Ai× Yoshikawa, Aoi |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Quality control in the process of preparation for urinary sediment samples is important for accurate diagnosis. In Japan, hematuria is defined as the presence of occult blood and five or more red blood cells per high power field in urinary sediment examination. The Japanese guidelines on urinary sediment examination procedures state that a urine sample should be centrifuged for 5 minutes at 500×g in the preparation process. Consequently, remaining components in the supernatant after centrifugation, especially suspended red blood cells, have been reported. However, changes in remaining red blood cells have not been investigated under different centrifugal conditions. We have investigated the percentage of remaining red blood cells in the supernatant, an issue in the guidelines on urinary sediment examination procedures, using artificial hematuria. Experiment 1: To investigate the percentage of remaining red blood cells with different centrifugal forces, we prepared two types of artificial hematuria with pseudo - urine made of saline and that made of 5% dextrose in acetated Ringer’s solution and examined the percentage of remaining red blood cells in the supernatant after centrifugation at 400 to 900×g. Although 3%-5% of the red blood cells remained at 500 ×g specified by the standard guideline, the percentage decreased to 2.0% or less at 700 ×g or higher. xperiment 2: Regarding the percentage of remaining red blood cells at different hours, the percentage was 6.87% for 5 minutes, 0.38% for 10 minutes, and 0.23% for 15 minutes at 500×g in artificial hematuria with pseudo-urine made of saline. This study suggests that a certain percentage of red blood cells is suspended in the supernatant by following the current Japanese guidelines on urinary sediment examination procedures, which could affect the number of red blood cells in urinary sediment examination. |
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| 書誌情報 |
北陸大学紀要 en : Bulletin of Hokuriku University 号 51, p. 135-141, 発行日 2021-09-30 |
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| 出版者 | ||||||
| 出版者 | 北陸大学 | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 2186-3989 | |||||
| 権利 | ||||||
| 権利情報 | 〔査読有り〕の原著論文 | |||||
